Enhanced degradation of high-concentrated 2,4,6 trichlorophenol-containing wastewater by micron-grade high iron-containing fly ash/loofah sponge biochar: Metagenomic functional succession

In order to strengthen the treatment of phenolic wastewater by anaerobic biotechnology, micron-grade high iron-containing fly ash/loofah sponge biochar (MHIFA/LsBC) was prepared and added to anaerobic activated sludge . It was domesticated and cultured in anaerobic bottle by a constant temperature oscillation incubator. Its promoting effect on the anaerobic degradation of high-concentrated 2,4,6-trichlorophenol (2,4,6-TCP) wastewater and its effect on the composition and function of microbial community were studied. The results indicated that with MHIFA/LsBC, the final removal rates of COD and 2,4,6-TCP in R1 (75.6% and 75.1%) were 2.1 and 1.81 times than those of the control group, respectively. The maximum methane yield was 107.25 mL/d. MHIFA/LsBC addition resulted in higher biomass and conductivity, and simultaneously increased the concentration of EPS and coenzyme F 420 . Microbial community analysis indicated that the enriched electrogenic bacteria Pseudomonas might perform DIET with Methanothrix through bioelectrical connection assisted by MHIFA/LsBC, and promote the anaerobic degradation of 2,4,6-TCP. As the functional genus induced by MHIFA/LsBC, Pseudomonas promoted the degradation and transformation of phenolic pollutants, and Azotobacter played a keystone role in metabolism for hydrogen production by preserving the NADH/NAD + balance.