A High-Sensitivity Coumarin-Based Fluorescent Probe for Monitoring Hydrogen Sulfide in Living Cells

A novel coumarin-based fluorescence probe has been constructed for the selective and sensitive detection of hydrogen sulfide (H 2 S). This probe displays high sensitivity and linearity to H 2 S in degassed PBS buffers and fetal bovine serum. It reacts with H 2 S with high selectivity over Cys, GSH, and other anions. Meanwhile, successful detection of H 2 S in living cells was also demonstrated.