Promoting Nanozyme Cascade Bioplatform by ZIF-Derived N-Doped Porous Carbon Nanosheet-based Protein/Bimetallic Nanoparticles for Tandem Catalysis

Nanozymes are a class of nanomaterials with enzyme-like activities that have been extensively studied for their possibilities and superiorities over natural enzymes. Intelligent design and fabrication of a nanozyme platform with highly efficient and stable tandem enzyme characteristics is necessary. Herein, a nanozyme cascade promoting bioplatform based on protein/bimetallic nanoparticles attached to ZIF-derived multilayer N-doped carbon nanosheets is proposed. Due to the carbon-based large surface area and solitary pairs of nitrogen electrons, the metal could be riveted to the carbon-based surface and reduced in situ to nanoparticles. Simultaneously, the addition of BSA has made it possible to establish a biological platform. The obtained BSA-PtAu@CNS has a linear range of 2 × 10–6 to 2 × 10–3 M for H2O2 and apparent dynamic Km of 0.005 × 10–3 M, which shows stronger affinity with H2O2 than HRP. The nanohybrid could adapt to severe reaction environment and enable prominent tandem dual-enzyme activity for glucose sensing. As a result, the cascade nanozyme bioplatform based on BSA-PtAu@CNS is accomplished and provides insights for subsequent biocompatible nanomaterials for use in medical and diagnostic applications.