Stereoselective interaction between hemoglobin and penicillamine enantiomers modified chiral surfaces

Chiral surfaces were obtained based on self-assembled monolayers of penicillamine enantiomers (L- or D-Pen) onto gold electrodes, and the enantioselective behavior was investigated by adsorbing hemoglobin (Hb) on the different chiral surfaces. Electrochemical methods, UV-vis and atomic force microscopy were adopted to monitor the protein adsorption. The properties of Hb adhesion on different chiral surfaces were further demonstrated by electrocatalytic responding to H2O2. The results showed that L-Pen had a higher dense adsorption to Hb than that of D-Pen. The Michaelis–Menten constants of the Hb/L-Pen/Au and Hb/D-Pen/Au sensors were first calculated to appeal to chiral recognition, the results showed that Hb/L-Pen/Au had a stronger catalytic ability, and further certified that Hb could adsorb on L-Pen assembled surfaces more easily. This work provided a promising reference for the investigation of chiral molecules.