Intrinsically fluorescent and highly functionalized polymer nanoparticles as probes for the detection of zinc and pyrophosphate ions in rabbit serum samples

Intrinsically fluorescent polymer nanoparticles (F-PNPs) were synthetized from 2-hydroxy-5-methylisophthalaldehyde and melamine by solvothermal method . F-PNPs can emit strong yellow green fluorescence at 542 nm without the conjugation to any external fluorescent agent and surface modification. Owing to the abundant amino and hydroxyl groups on their surface, the F-PNPs possess multiple binding sites, good biocompatibility and excellent water-solubility. Addition of Zn 2+ to the F-PNPs solution resulted in a blue shift (Δλ=40 nm) with obvious enhancement in the fluorescence intensity at 502 nm; while there was negligible change in the presence of other metal ions . The subsequent treatment with pyrophosphate (PPi) can cause fluorescence recovery of F-PNPs by pulling the Zn 2+ out of the coordination cavity of F-PNPs-Zn 2+ nanocomposites. No interference was observed from other anions and nucleotides, making the F-PNPs-Zn 2+ ensembles highly sensitive and selective nanoprobes for PPi. The detection limit is 2.75 × 10 −8 M/L and 7.63 × 10 −8 M/L for Zn 2+ and PPi, respectively. The proposed nanoprobes were then used for detecting the recovery of Zn 2+ and PPi in rabbit serum samples, which were found to be 99.4–104.2% and 98.6–104.7%, respectively. The present strategy for the fabrication of nanoparticles may offer a new sight for the preparation of polymer nanostructures . The F-FNPs based probes can provide an accurate method for the detection of Zn 2+ and PPi in serum samples.