Preparation and Performance of Polyvinyl Butyral Nanofiber Membrane for Lipase Immobilization

A type of nanofiber membrane of polyvinyl butyral (PVB)/OP-10/lipase was prepared using electrostatic spinning technology. The results of environmental scanning electron microscopy (ESEM) indicate that the microscopic morphology of PVB/OP-10/lipase membrane spun under 14 kV of voltages, 10 %wt concentration of emulsifier (relative to the amount of PVB) and 10 cm of the distance from the receiver to the needlepoint is uniform. The stability of immobilized and free lipase was studied under different pH, voltages and temperature and storage time. It is showed compared with the free enzyme, the immobilized enzyme has better temperature-resistant and better storage stability. The immobilized enzyme has recycling activity of 65% after 10 times uses. The esterification rate of lauric acid with lauryl alcohol catalyzed by immobilized lipase can get to 72% after 20 hours of reaction. While the reaction of lauric acid with lauryl alcohol catalyzed by free lipase cannot occur.