Natural Deep Eutectic Solvents as Performance Additives for Peroxygenase Catalysis

Graphical Natural Deep Eutectic Solvents : Envisioned bienzymatic cascade for the selective oxyfunctionalisation of ethyl benzene or cis-ß-methylstyrene comprising the evolved peroxygenase from Agrocybe aegerita (r Aae UPO) and choline oxidase from Arthrobacter nicotianae ( An ChOx) to provide r Aae UPO with H 2 O 2 . Natural deep eutectic solvents (NADES) are proposed as alternative solvents for peroxygenase-catalysed oxyfunctionalization reactions. Choline chloride-based NADES are of particular interest as they can serve as solvent, enzyme-stabiliser and sacrificial electron donor for the in situ H 2 O 2 generation. This report provides the first proof-of-concept and basic characterisation of this new reaction system. Highly promising turnover numbers for the biocatalysts of up to 200,000 have been achieved.