Preparation and Characterization of Active Targeting Dual-Functionalized Liposome with Anti-Tumor Ability and Optimized Fluorescent Intensity

Although the preparation of Indocyanine Green (ICG) liposomes obtained stronger performance than free ICG. With increase in depth of tissue, ICG exhibits limited background (SBR) and blurs structure characteristics. In this research, a Stearylamine-Bearing cationic liposome was prepared for improved fluorescence performance (higher SBR and deeper imaging depth). In addition, the effect of ICG and lipid interactions was explored. Hyaluronic acid is subsequently modified on the liposomes for prolonging blood circulation time and active tumor targeting. In vitro study confirmed that the liposome (HA-ICG-SA-LP) was capable of reversing surface zeta potential under acidic conditions in the presence of HAase which might enhance cellular uptake. Additionally, the photothermal heating of liposomes was investigated. The MTT assay showed that the liposome has strong cancer cell inhibition ability. In summary, HA-ICG-SA-LP exhibited a great potential for high sensitivity imaging and tumor hyperthermia.