Metabolic profiling of antioxidants constituents in Artemisia selengensis leaves

This study aimed to evaluate the antioxidant potential of Artemisia selengensis Turcz (AST) leaves, a byproduct when processing AST stalk, and identify the antioxidant constituents by using HPLC-QTOF-MS 2 . The total phenolics content (TPC), total flavonoids content (TFC) and antioxidant abilities of fractions resulted from the successively partition of chloroform, ethyl acetate and n -butanol were compared. Ethyl acetate fraction (EAF) exhibited the highest TFC (65.44   mg   QuE/g   fraction), n -butanol fraction ( n BuF) showed the highest TPC (384.78   mg   GAE/g   fraction) and the best DPPH scavenging ability, ABTS + scavenging ability and reducing power. Totally, 57 compounds were identified or tentatively identified in n BuF and EAF, 40 of them were reported in AST for the first time. The major constituents in EAF were flavonoids, and the major constituents in n BuF were phenolic acids and organic acids. Thus, AST leaves might be a potential low-cost resource of natural antioxidants .