Reducing β-glucosidase supplementation during cellulase recovery using engineered strain for successive lignocellulose bioconversion

Enzyme recycling by re-adsorption is one of the primary methods for reducing enzyme usage in lignocellulose conversion. This work proposes the combination of an engineered yeast strain that expresses β-glucosidase with enzyme recycling to reduce the amount of supplemented β-glucosidase in enzyme recycling experiments. Using the engineered strain, a slight increase in ethanol concentration was obtained after a 96-h fermentation of pretreated corncobs. Ethanol concentrations increased by 34.7% and 62.7% in the following two recycle rounds using the engineered strain compared with those using its parental strain without β-glucosidase addition. Furthermore, with the addition of β-glucosidase at 30   CBU/g cellulose, the ethanol concentration after two recycle rounds exceeded 90% of that observed in the first SSF round with the engineered strain at a high initial cellulase loading of 45   FPU/g cellulose.