Iminocoumarin-based fluorescence probe for intracellular H2O2 detection with a red emission and a large Stokes shift

A highly sensitive and selective fluorescence probe for H 2 O 2 has been developed based on an iminocoumarin derivative using aromatic boronic ester as sensing unit. A significant fluorescence enhancement was observed with λ max em = 619 nm when this probe was treated with H 2 O 2 . In the detection of H 2 O 2 , this probe exhibited a large Stokes shift (148 nm) and a low detection limit (6.0 × 10 −8  mol/L). Furthermore, cell imaging studies had revealed that this probe had the potential to detect intracellular H 2 O 2 in living cells.