A simple and effective coumarin-based fluorescent probe for cysteine

Acrylic acid 3-acetyl-2-oxo-2 H-chromen-7-yl ester ( ACA ) was rationally designed and synthesized as a simple and effective fluorescent probe for sensing cysteine with high selectivity and naked-eye detection. The probe can detect cysteine by fluorescence spectrometry with a detection limit of 0.657   μM and can be used with calf serum and in live cell imaging . The conjugate addition/cyclization sequence mechanism of the reaction between ACA and cysteine was confirmed by ESI-MS and fluorescence spectra .