Novel coumarin-based containing denrons selective fluorescent chemosesor for sequential recognition of Cu2+ and PPi

In this study, we have successfully synthesized a novel coumarin-based dendrons derivative CD and its chemical structure was characterized by 1 H NMR, 13 C NMR and ESI-HR-MS. The sensor CD showed an obvious “on-off” fluorescence quenching response toward Cu 2+ with a maximum quenching efficiency of 99.8%. The CD -Cu 2+ complex showed an “off-on” fluorescence enhancement response toward PPi over many competitive anions. The detection limit of the sensor CD was 0.29 × 10 −6  M to Cu 2+ and 2.39 × 10 −9  M to PPi. In addition, the sensor CD showed a 1:1 binding stoichiometry to Cu 2+ and the sensor CD -Cu 2+ showed a 2:1 binding stoichiometry to PPi in CH 3 CN/HEPES buffer medium (9:1 v/v, pH = 7.2). The stable pH range of sensor CD to Cu 2+ and CD -Cu 2+ to PPi was from 3 to 8.