Fabrication of Quercitrin Nano Micellar Delivery System and Its Therapeutic Effect on Unexplained Recurrent Abortion

We prepared a quercitrin nano micellar delivery system in this study to evaluate its oral bioavailability. The optimal formulation of quercetin nano micelles was determined through an orthogonal test. Characteristics (size of particles-SOP, morphology, efficiency of encapsulation-EE and stability) and the therapeutic property of quercitrin nano micelles on unexplained recurrent abortion (URSA) were evaluated. The SOP of quercitrin nano micelles was 111.88±3.70 nm with an EE of 95.66±0.57. A substantially increased release rate of quercetin from the micellar system was observed in different dissolution media comparable to that of quercitrin. Also, through quercitrin micelles, the oral bioavailability of quercetin was increased by 15.45-fold compared to quercitrin solution. Significantly, quercetin could reduce the levels of LDH and SOD as well as increase the level of MDA in serum restricted HTR-8/SVneo cells. Western blotting (WB) experiments showed that quercitrin had a protective effect on H2O2 induced oxidative stress injury of a human placental trophoblast HTR8-SVneo cell line. The developed nano micelles are a potential carrier that could enhance the aqueous solubility, oral in vivo availability and potential therapeutic abortion effect of quercitrin.