Simultaneous Determination of Cyclamate, Acesulfame, and Aspartame in Beverages by Titania-Based RP-HPLC

This paper aims at establishing a rapid method for simultaneous separation and determination of the sweeteners including cyclamate, acesulfame, and aspartame in beverages by titania-based reversed-phase high-performance liquid chromatography. The chromatographic conditions were as follows: a titania Sachtopore-RP C18 column (250 × 4.6 mm; 5 μm) as a separation column, a mixture of water and methanol at a volume ratio of 95:5 containing 1.0 % phosphate acid used as mobile phase, flow rate of 1.0 mL min −1 , and the detection wavelength was 205 nm. The linear ranges of cyclamate and aspartame were 0.02–8.0 mg mL −1 with limits of detection (LODs) of 16.35 and 19.56 μg mL −1 , respectively, and their limits of quantitation (LOQs) were 55.50 and 68.50 μg mL −1 , respectively. The recoveries of cyclamate were between 93.52 and 103.54 %. The recoveries of aspartame were between 93.31 and 102.63 %. The linear range of acesulfame was 0.125–50 μg mL −1 with LOD of 0.08 μg mL −1 and LOQ of 0.25 μg mL −1 . The recoveries of acesulfame were between 94.34 and 103.21 %. Relative standard deviation ( n  = 8) for all determinations was less than 0.72 %.