Facile Preparation of Activated Carbon from Peanut Shell for Determination of Bisphenol A in Human Urine by High-Performance Liquid Chromatography

Herein, a facile and low-cost method for the preparation of activated carbon from peanut shell was developed for the first time for the fast extraction and determination of Bisphenol A in human urine. Bisphenol A was separated by EC-C18 column (250 mm×4.6 mm, 4 μm) and was detected by VWD, with retention time for qualitative analysis and peak area for quantitation. The parameters, pH values of the urine, adsorbent dose, adsorption time and so on, were optimized to achieve the excellent extraction performance. The detection limit of Bisphenol A in human urine was 1.0 ng · mL−1 (S/N = 3), and the standard curve was linear in the range of 5.0 ng · mL−1˜200.0 ng · mL−1 (r = 0.9993). The average recovery of Bisphenol A was 78.5˜96.2% at three spiked levels in the range of 5.00 ng · mL−1˜200.00 ng·mL−1. The method was proved simple, practical and highly sensitive, which could satisfy the request for the determination of Bisphenol A in human urine.