Colorimetric detection of α-glucosidase activity based on the etching of gold nanorods and its application to screen anti-diabetic drugs

An ultrasensitive colorimetric method for α-glucosidase sensing based on iodine-mediated etching of gold nanorods (AuNRs) was developed. In this method, 2- O -α- d -glucopyranosyl- l -ascorbic acid (AA-2G) was used as an enzymatic substrate. The hydrolysate ascorbic acid in situ reduced KIO 3 to I 2 , which subsequently etched the AuNRs. A blue-shift of the localized surface plasmon resonance (LSPR) absorption of AuNRs was clearly observed by UV–vis spectroscopy and reflected with visual color changes. The shift showed a good linear relationship with the concentration of α-glucosidase during 2.5–45 U/L and a low α-glucosidase detection limit of 0.5 U/L was provided. This strategy was featured with low cost, simplicity, excellent sensitivity, high selectivity and successfully applied in the α-glucosidase inhibitor screening with Chinese Wolfberries as candidates.