Fe3O4@MoS2@PEI-facilitated enzyme tethering for efficient removal of persistent organic pollutants in water

A kind of core-shell material with regular shape and size, and uniform composition, is prepared in aqueous phase and used for laccase immobilization. Magnetic Fe 3 O 4 nanoparticle is coated with MoS 2 by self-assembly, and the Fe 3 O 4 @MoS 2 is further coated by polyethyleneimine, which acts as a spacer arm and is beneficial to enzyme immobilization. The nanocarrier affords the high loading capability of 120 mg/g and satisfied activity recovery of 90% for laccase. The immobilized laccase is stable in a wider range of pH (3–10), temperature (20–70 °C) and storage time (1–14 days), as well as having the recyclability (1–10 cycles). The carcinogenic persistent organic pollutants (malachite green, bisphenol A, bisphenol F) in the water are degraded by the immobilized laccase with a higher efficiency (82.7%, 87.6%, 70.6%) than free laccase (14.4%, 85.0%, 48.0%), respectively. It is prospected that the constructed material is qualified as green nanoreactor for biological macromolecules immobilization.