A novel glucose colorimetric sensor based on intrinsic peroxidase-like activity of C60-carboxyfullerenes

C 60 -carboxyfullerene, C 60 [C(COOH) 2 ] 2 , has been proven to function as peroxidase mimetics that can catalyze the reaction of peroxidase substrate 3,3',5,5'-tetramethylbenzidine (TMB) in the presence of H 2 O 2 to produce a blue color reaction. Kinetic studies further indicate that C 60 [C(COOH) 2 ] 2 has an even higher affinity to TMB than that of the natural enzyme , horseradish peroxidase (HRP). C 60 [C(COOH) 2 ] 2 /glucose oxidase (GO x )/TMB system provides a novel colorimetric sensor for glucose and shows good response toward glucose detection over a range of 1.0–40   μM with a limit of detection 0.5   μM (3 σ /slope). Moreover, this sensitive and selective sensor can be successfully applied for the quantitative determination of glucose in human serum. The results indicate that it is a simple, cheap, convenient, highly selective, sensitive, and easy handling colorimetric assay .