One-pot preparation of an acryloyled β-cyclodextrin-silica hybrid monolithic column and its application for determination of carbendazim and carbaryl

This work describes, for the first time, an acryloyled β -cyclodextrin hybrid monolith column was synthesized, under aqueous-phase conditions, and used for solid-phase microextraction of carbendazim and carbaryl . The monolithic column was characterized using scanning electron microscopy, nitrogen adsorption–desorption, thermogravimetric analysis and Fourier transform infrared spectroscopy, and used as the adsorbent for solid phase microextraction (SPME) of carbendazim and carbaryl. After optimization of the SPME conditions, a simple and sensitive SPME-HPLC method was developed for the determination of carbendazim and carbaryl in leafy vegetables. The method exhibited a good liner response in the range 5–400 μg/kg (R 2  = 0.9994) for carbendazim and 10–400 μg/kg (R 2  = 0.9996) for carbaryl, respectively. The limits of detection were 1.0 and 1.5 μg/kg for carbendazim and carbaryl, respectively, in leafy vegetables. Recoveries ranged from 92.6% to 110.1%, and the relative standard deviations were less than 6.1%.