A new pyrazoline-based probe of quenched fluorescent reversible recognition for Cu2 + and its application in cells

A new pyrazoline-based probe D was designed and synthesized, which can be used as a highly sensitive, selective and reversible recognizing fluorescent to detect Cu 2   + . The recognition properties of this compound was investigated by UV–vis absorption and fluorescence spectrophotometry. The results showed that the probe D forms a 1:1 complex with Cu 2   + and displayed a linear fluorescence response to Cu 2   + with a detection limit of 1.94   ×   10 −   7   M. In addition, the probe have a good biocompatibility in living cells.