UV-Vis detection of hydrogen peroxide using horseradish peroxidase/copper phosphate hybrid nanoflowers

A colorimetric platform based on enzyme-inorganic hybrid nanoflowers was used for ultraviolet-visible detection of hydrogen peroxide (H 2 O 2 ). The hybrid nanoflowers were prepared via incubation at room temperature using horseradish peroxidase (HRP) and copper phosphate (Cu 3 (PO 4 ) 2 ) as the organic and inorganic components, respectively. HRP/Cu 3 (PO 4 ) 2 hybrid nanoflowers (HRP-HNFs) showed linear H 2 O 2 detection range from 100 nM to 100 μM, moreover, HRP-HNFs exhibited good selectivity for H 2 O 2 detection due to the specificity of HRP. In addition, HRP-HNFs have good reusability, excellent color stability and long-term storage stability, which indicate HRP-HNFs are promising for catalysis and biomedical applications.