Deposition of Au Nanoparticles onto Poly(3,4-ethylenedioxy - thiophene) Functionalized Multi-Walled Carbon Nanotubes for Label-Free Immunosensing

A high performance electrochemical immunosensor was constructed for sensitive label-free detection for α-fetoprotein (α-FP) based on Au nanoparticles/poly(3,4-ethylenedioxythiophene) modified multi-walled carbon nanotubes (AuNPs/PEDOT-MWCNTs), which acted as signal amplification matrix. PEDOT functionalized MWCNTs were prepared via interfacial polymerization method. PEDOT was uniformly distributed on MWCNTs, which can provide more binding sites for high mass loading of metal nanoparticles. Subsequently, AuNPs were attached on MWCNTs by introducing conductive PEDOT to bridge the AuNPs and MWCNTs walls. The AuNPs/PEDOT-MWCNTs nanocomposites displayed good electron transfer ability, which enabled good analytical properties for α-FP detection.