Analysis of optical properties and response mechanism of H2S fluorescent probe based on rhodamine derivatives

H 2 S plays a crucial role in numerous physiological and pathological processes. In this project, a new fluorescent probe, SG-H 2 S , for the detection of H 2 S, was developed by introducing the recognition group 2,4-dinitrophenyl ether. The combination of rhodamine derivatives can produce both colorimetric reactions and fluorescence reactions. Compared with the current H 2 S probes, the main advantages of SG-H 2 S are its wide pH range (5–9), fast response (30 min), and high selectivity in competitive species (including biological mercaptan). The probe SG-H 2 S has low cytotoxicity and has been successfully applied to imaging in MCF-7 cells, HeLa cells, and BALB/c nude mice. We hope that SG-H 2 S will provide a vital method for the field of biology.