Metabolic response of Citrus limon to Asian citrus psyllid infestation revealed by EESI-MS and HPLC

Asian citrus psyllid (ACP) causes direct and indirect damage to the citrus industry. Extractive electrospray ionization mass spectrometry (EESI-MS) and high performance liquid chromatography (HPLC) were used to detect the metabolites of C. limon leaves at 0, 12, 24, and 72 h after ACP treatment. The EESI-MS results showed that ACP infestation significantly affected metabolites within a short feeding duration with 8 metabolites identified. The metabolites in leaves of these four groups could be distinguished, with 55 peaks showing significant differences including methyl N -methylanthranilate, caffeic acid , and syringic acid . The quantification of 15 phenolic compounds with HPLC-UV method in C. limon leaves after ACP infestation showed that the total content of them reached a peak of 3504.69 μg g −1 at 12 h, with 9 phenolic compounds changing significantly ( P  < 0.05). A total of 21 metabolites identified in this study were involved in the biosynthesis pathways of flavonoid , flavone and flavonol , isoflavonoid and phenylpropanoid , and the degradation of aminobenzoate . Contents of epicatechin and caffeic acid increased with the feeding time of ACP as detected by both EESI-MS and HPLC. This may be related to plant defense. This study provides novel insights into the biochemical relationship of ACP and its host plants.