Synthesis of monodisperse magnetic restricted microspheres for recognition of thiamphenicol in milk

Taking thiamphenicol as the research object, a new type of magnetic restricted access molecularly imprinted polymer (RAM-MMIP) with specific recognition was prepared by a one-step swelling method. The polymer microspheres were characterized and analyzed by scanning electron microscopy, X-ray diffraction, elemental analysis, contact angle measurement and vibrating sample magnetometry. When the ratio of template molecule, functional monomer and cross linking agent was 1 : 4 : 8, the adsorption capacity reached the maximum. Under these conditions, RAM-MIP magnetic solid phase extraction (M-SPE) was combined with HPLC to analyze thiamphenicol in milk samples. Satisfactory linear correlation (R2 > 0.9977), good detection limit (LOD: 10.4 μg L−1), high recovery rate (96.5–101.1%), and relative standard deviation (RSD: 2.8–3.8%) were obtained. Therefore, our synthesized material can be used for the analysis of TAP in complex milk samples, and has broad application value.