A ratiometric water-soluble fluorescent probe for the detection of sulfur dioxide derivative in sinusitis mice model

Sulfur dioxide (SO 2 ) plays an extremely important role in the basic processes of physiology and pathology. As an antioxidant , SO 2 can maintain the redox homeostasis in the cell. Excessive inhalation of SO 2 would lead to irreparable respiratory damage, resulting in respiratory diseases , neurological disorders , and even cardiovascular disease. Thus, it is urgent to exploit an excellent way to monitor SO 2 derivatives in biological system. Herein, we design a water-soluble ratiometric fluorescent probe to fast detect the level of SO 2 derivatives in living cells in vivo. The probe displays obvious fluorescence signal at long wavelength, which is helpful for imaging of biological system. After respond to SO 2 derivatives, the fluorescence signal at 465 nm increases rapidly due to the Michael addition reaction is triggered, further causing the disruption of large conjugated system . The probe exhibits high selectivity and fast respond to SO 2 derivatives, which can be able to sensitive and real-time monitoring of SO 2 derivatives level in living cells. Moreover, the probe reveals a low detection limit and a great linear relationship to SO 2 derivatives. Based on the negligible cytotoxicity and good biocompatibility of the probe, which is employed to detect exogenous and endogenous SO 2 derivatives in living cells. In addition, it is also served as a potential chemical tool to detect SO 2 derivatives in mice model of sinusitis .