Feasibility for quantitative determination of deoxyribonucleic acid by using near-infrared diffuse reflectance spectroscopy

A method for quantitative determination of fish sperm deoxyribonucleic acid (fsDNA) in solutions was developed by using adsorption preconcentration and near-infrared diffuse reflectance spectroscopy (NIRDRS). A high capacity adsorbent of amino-modified silica particle (AMSP) was prepared for preconcentration of fsDNA in solutions. Under the optimized conditions, the adsorption rate can be above 90% within 3   min. After adsorbing the DNA onto the adsorbent, near-infrared (NIR) spectra in diffuse reflectance mode were measured and partial least squares (PLS) model was established for fast quantitative prediction. The results show that the correlation coefficient ( R ) between the predicted and the reference concentration is 0.9894 and the recoveries are in the range of 92.9–123.4% for the validation samples in the concentration range of 3.00–29.38   mg   L −1 . Therefore, the feasibility for quantitative analysis of DNA in solutions by NIRDRS is proved. This may provide an alternative way for fast determination of DNA in solutions.