Development of the detection of benzophenone in recycled paper packaging materials by ELISA

An enzyme-linked immunosorbent assay method was developed here for benzophenone (BZP) detection in package paper samples based on polyclonal antibody. 4-Aminobenzophenone (ABZP) was used as hapten and conjugated with bovine serum albumin for antibody production. Cross-reactivity results showed that the antibody can be recognised BZP, ABZP and Michler's ketone very well (IC50 value was 31.2±7.8 ng/mL, 35.9±8.3 ng/mL and 43.7±8.2 ng/mL, respectively). After optimisation of the effects from pH, ionic strength and organic solvents on immunoassay, we established a rapid method and used for BZP detection in fortified package paper samples with good recovery data (98.5 and 99.7%) at 100 and 600 ng/g spiked level.)