Paenibacillus campi sp. nov., a novel endophytic bacterium isolated from Pennisetum species

Two facultatively aerobic, Gram-stain-positive, rod-shaped, endospore-forming, endophytic bacteria, designated SGZ-1009Tand SGZ-1014, were isolated from the plantPennisetumsp. Strain SGZ-1009Tgrew at 5–50 °C, pH 4.5–11.0 and tolerated up to 4.5% NaCl (w/v), while strain SGZ-1014 exhibited growth at a temperature range of 10–50 °C, pH range 4.5–11.0 and tolerated up to 4.5% NaCl. Phylogenetic analysis based on 16S rRNA gene sequences indicated that these two strains belong to the genusPaenibacillus, closely related to the reference type strain ofPaenibacillus wenxiniaeDSM 100576T(similarity of 97.0%). The genomic relatedness values for both strains, SGZ-1009Tand SGZ-1014, compared to their closest reference strainP. wenxiniaeDSM 100576T, were 81.6% for average nucleotide identity and 26.0% for digital DNA–DNA hybridization, suggesting strain SGZ-1009Trepresents a novel species. The genomic DNA G+C content of strain SGZ-1009Twas 46.4%. Both strains shared anteiso-C15:0(55.9%) as the predominant fatty acid, menaquinone-7 as the major respiratory quinone and diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine as the major polar lipids. Based on these results, strains SGZ-1009Tand SGZ-1014 represent a novel species ofPaenibacillus, for which the namePaenibacillus campisp. nov. is proposed. The type strain is SGZ-1009T(=MCCC 1K08892T=GDMCC 1.4280T=KCTC 43677T=JCM 36670T).