Copper-doped Lanthanide Coordination Polymers as Luminescent Nanoenzyme for Ratiometric Sensing of H2O2 and Glutathione

Design and fabrication of integrated multifunctional probes with intrinsic catalytic and detection abilities is of great importance to simplify the operation in biosensing application with high sensitivity. Herein, dual-emitting lanthanide coordination polymers (Ln-CPs) were facilely prepared by self-assembly of guanine diphosphate (GDP), terephthalic acid (TA), Tb 3+ and Cu 2+ designated as Tb/Cu-GDP/TA CPs. The doped Cu 2+ endowed CPs with obviously enhanced peroxidase mimicking activity compared with free Cu 2+ . In the presence of H 2 O 2 , the probe catalyzed the oxidation of TA generating a new blue fluorescent product, while the fluorescence of Tb 3+ decreased simultaneously. Therefore, a new sensitive ratiometric fluorescent sensor for H 2 O 2 has been developed with a good linear range from 0.01 to 300 μM and limit of 1.62 nM. Moreover, the proposed platform could be extended to GSH ratiometric assay in the presence of H 2 O 2 , and interestingly, the detection performance could be easily adjusted by adding different concentration of H 2 O 2 . This work will facilitate the development of luminescent nanoenzymes based on Ln-CPs to construct the simple ratiomatric sensing platform.