Ancylobacter terrae sp. nov. and Ensifer soli sp. nov., two novel bacterial species isolated from paddy soil

Two novel aerobic, rod-shaped, non-motile and white-coloured bacterial strains, designated as sgz301288Tand sgz301302T, were isolated from paddy soil in Fujian Province. 16S rRNA gene phylogenetic analysis revealed that strain sgz301288Texhibited 97.2% similarity withAncylobacter moscoviensisKCTC 62336T, while strain sgz301302Thad the highest 16S rRNA gene sequence similarity toCiceribacter azotifigensKACC 19080T(97.4%) andEnsifer terangaeLMG 7834T(97.0%). Average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values between sgz301288TandA. moscoviensisKCTC 62336Twere 79.5% and 22.9%, respectively. For strain sgz301302T, ANI and dDDH values were 76.7% and 21.5% withE. terangaeLMG 7834Tand 75.9% and 20.8% withC. azotifigensKACC 19080T. Phylogenomic analysis further supported strain sgz301288Tbelonging toAncylobacter, whereas sgz301302Tclustered withinEnsifer, distinct fromCiceribacter. The DNA G+C contents of sgz301288Tand sgz301302Twere 68.4% and 66.5 %, respectively. Strain sgz301288Tgrew at 20–30 °C (optimum 30 °C) and pH 6.5–9.5 (optimum 7.0) and tolerated up to 0–1.0% NaCl, while strain sgz301302Tgrew at 20–37 °C (optimum 30 °C), pH 5.0–9.5 (optimum 7.0) and 0–1.5 % NaCl. Both strains contained ubiquinone-10 as the predominant respiratory quinone. The main fatty acids were iso-C17 : 03-OH and summed feature 8 (C18 : 1ω7cand/or C18 : 1ω6c) for sgz301288Tand C16 : 0and summed feature 8 for sgz301302T. Based on the polyphasic evidence, the proposed names of the two novel species areAncylobacter terraesp. nov. (type strain sgz301288T=MCCC 1K09301T=KCTC 8858T) andEnsifer solisp. nov. (type strain sgz301302T=MCCC 1K09182T=KCTC 8857T), respectively.