pH-responsive star-shaped functionalized poly(ethylene glycol)-b-poly(ε-caprolactone) with amino groups

Functional star-shaped 4-arm poly(ethylene glycol)- b -poly[( ε -caprolactone- co - γ -amino- ε -caprolactone)] (4-arm PEG- b -P(CL- co -ACL) was synthesized through ring-opening polymerization. The structure of the copolymer was confirmed by 1 H NMR, Fourier transform infrared spectroscopy (FTIR), and gel permeation chromatography (GPC). To further understand the copolymers, the difference of the conversion rate between ε -caprolactone (CL) and γ -(carbamic acid benzyl ester)- ε -caprolactone (CABCL) and the detailed deprotection condition were studied. The thermal property of the copolymer was analyzed by WAXR and differential scanning calorimetry (DSC), which demonstrated that the thermal property could be well adjusted. The pH-responsive behavior of the copolymers was studied in detail by dynamic light scattering (DLS), pH titration, and pyrene fluorescence methods, which indicated that it could form micelles and exhibit pH responsibility. Moreover, the copolymer was nontoxic and had good biocompatibility according to the results by 3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-di-phenytetrazoliumromide (MTT) assay.