A 384-Site Chip Platform for Biochemical Applications With Individual Site Precision Temperature Control

A chip platform for thermally controlled biochemistry is presented comprising postprocessed thermally isolated heated reaction sites atop a application-specific integrated circuit (ASIC) using proportional-integral-differential (PID) feedback temperature control. The 384 sites can be individually programmed over $25~^{\circ }$ C– $125~^{\circ }$ C, with thermal crosstalk below the $\pm 0.12~^{\circ }$ C precision. Double-strand deoxyribonucleic-acid (DNA) purification via selective melting is demonstrated, with 70% retention of perfectly matched sequences, while 55% of those with two mismatched base-pairs are removed.