Optimization of Fabrication Parameters to Prepare Tea Catechin-Loaded Liposomes by Fuzzy Orthogonality

The fuzzy orthogonal design is a method that the orthogonal results were fuzzed and then analyzed using fuzzy mathematical theory. It took the full advantage of the information from orthogonal experiments to obtain the optimal liposome preparation conditions by considering all the evaluation indexes. With the fuzzy comprehensive evaluation for the objective function, main technological parameters of process in liposome preparation were optimized using fuzzy orthogonal design. The optimal processing parameters were pH values of phosphate-buffer solution of 7,soybean phosphatidyl-choline and cholesterol proportion of 1:1,the concentration of Catechin of 5mg/mL,Oil­water proportion of 3:1.The catechin-loaded liposomes prepared under the optimized conditions has high encapsulation efficiency. The entrapment rate of catechin nanoliposome measured by validated test was 61.7%±0.015,while the mean diameter was 193.6±0.119 nm.