Spectroscopic investigations on the binding of leucomalachite green to bovine serum album

The interaction of bovine serum with leucomalachite green (LMG) was investigated by spectroscopic methods . LMG was found to quench the intrinsic fluorescence of BSA by dynamic quenching. High quenching constant suggested that LMG can form a complex with BSA. Binding constant and number of binding site were determined. The thermodynamic parameters suggested the main interacting force is hydrophobic forces . The effect of LMG on the conformation of BSA was analyzed by synchronous fluorescence spectroscopy . RLS spectra were also used to reconfirm the formation of complex between BSA and LMG.