Fabrication of Myoglobin-Sodium Alginate-Graphene Composite Modified Carbon ionic liquid electrode via the Electrodeposition Method and Its Electrocatalysis toward Trichloroacetic Acid

To achieve the accurate determination of trichloroacetic acid (TCA) in water chlorination, the sensitive electrochemical biosensor, Nafion/myoglobin-sodium alginate-graphene composite modified carbon ionic liquid electrode (Nafion/Mb-SA-GR/CILE), was fabricated by the electrodeposition method. Spectroscopic results implied that Mb could keep its biological activity in the SA-GR composite film. Direct electrochemistry of Nafion/Mb-SA-GR/CILE indicated that direct electron transfer of Mb was realized on the modified electrode, which was attributed to the synergistic effects of SA-GR composite film with excellent electrocatalytic capability and good biocompatibility. Furthermore, the modified electrode displayed good electrocatalytic ability towards TCA with the linear range from 7.5 to 69.0 mmol/L and a low detection limit of 0.163 mmol/L (3σ). Meanwhile, the modified electrode possessed good stability, reproducibility and excellent accuracy for the detection of tap-water, exhibiting great potentials for electroanalysis and electrocatalysis applications.