Fatty acid composition and nutritional analysis of waste crude fish oil obtained by optimized milder extraction methods

The increasing environmental impact of fish waste has attracted more attention towards developing simple, greener, and effective valorization methods. This study employed optimized milder extraction methods as a potential process intensification strategy to valorize marine and freshwater fish wastes with significant proximate compositions (p < 0.05). Using response surface methodology, the optimized parameters, including lower temperature (60℃), less enzyme concentration (1%), and 1:1 ratio of less toxic solvents, had high desirability levels (˃ 0.92). Although identical chemical functional groups were observed in the infrared spectra, the marine fish waste produced higher oil yields. The physicochemical characteristics of the oils were within the international CXS 329-2017 standard. Palmitic acid (21.21–26.63%), oleic acid (19.78–27.11%), eicosapentaenoic acid (6.02–9.97%), and docosahexaenoic acid (17.00- 20.83%) were the dominant saturated and unsaturated fatty acids in the cr...