The Interference of Mnsod3 Enhances the Tolerance of Pleurotus ostreatus Mycelia to Abiotic Stress by Reshaping the Cell Wall

In recent years, the response mechanism ofPleurotus ostreatusto abiotic stress has received widespread attention. MnSOD is an important antioxidant enzyme that has been widely studied in animals and plants because of its functions. However, there is little research on the function and regulatory mechanism of MnSOD in the growth and development of edible fungi. This study investigated the role ofMnsod3in the growth and development ofP. ostreatus. The results showed that during the nutritional growth stage, heat stress can cause the cell wall of mycelia to shrink and the cells to exhibit cytoplasmic wall separation. RNA-seq revealed thatMnsod3interference is strongly correlated with increased transcript levels of cell wall synthase genes and with increased tolerance to cell wall disruptors. During the primordium formation stage, the mycelial cell wall also significantly wrinkled under cold and light stresses. RNAi ofMnsod3alleviated the cell wall wrinkling caused by cold and light stress, restored the smoothness of the cell walls, and increased mycelial tolerance to abiotic stress. This may be related to the slower formation rate of primordia, but the specific molecular mechanism still needs further research. and slowed the rate of primordium formation. In summary,Mnsod3plays an important role in the growth and development ofP. ostreatusunder abiotic stress and plays a critical regulatory role in cell wall remodeling under abiotic stress.