CuS@Pt@ZIF-8 mediated Fenton-like nanozyme for synergistic photothermal and chemodynamic antibacterial wound healing therapy

In nanomaterials containing multiple metal atoms, interactions between metal atoms can generate the Fenton effect, making them a novel antibacterial agent that combines photodynamic and chemodynamic properties. However, conventional single-atom or multi-atom nanomaterials are limited by their specific surface area and porosity, resulting in relatively low photocatalytic efficiency. Herein, we developed a nanzyme antibacterial agent based on a porous ZIF-8 metal-organic framework incorporated with Pt and CuS via a straightforward method. Under low-concentration near-infrared (NIR) light irradiation, the CuS enables the nanzyme to rapidly raise the reaction system temperature to about 50 °C within 5 min. Furthermore, facilitated by charge transfer between Cu and Pt metals and the prominent Fenton-like activity of Pt, the system efficiently catalyzes hydroxyl radical generation from trace amounts of H 2 O 2. This mechanism synergistically integrates photodynamic and chemodynamic actions for antibacterial efficacy. The designed CuS@Pt@ZIF-8 nanzyme achieves a bacterial elimination rate of over 95% against common bacteria when subjected to irradiation with 808 nm NIR light. In vivo experiments in mice demonstrated a wound healing rate of up to 98% within 7 days. Additionally, experiments on plants and animals demonstrated that this nanzyme has minimal toxicity. The broad-spectrum Fenton-like catalytic system provide an efficient strategy for drug-resistant bacterial wound infections under combined photodynamic and chemical dynamics therapy, offering a new avenue for the development of clinical drugs.