A Novel Species Description of Sphingobium agrisoil sp. nov.

Two Gram-stain positive, motile, aerobic, yellowish rod-shaped bacterial strains sgz301303 and sgz301304 T were isolated from the paddy soils. The 16S rRNA gene phylogenetic analysis revealed that both strains belong to the genus Sphingobium , and exhibited the highest sequence similarity (98.3%) with reference strain Sphingobium amiense JCM 11777 T . The draft genome of sgz301304 T has a size of 4.3 Mbp, with DNA G + C content of 64.5%. Whole-genome relatedness analyses showed that the average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain sgz301304 T and S. amiense JCM 11777 T were 82.3% and 25.7%, respectively, both below the accepted species delineation thresholds. The main quinone was Q-10, and the main fatty acids included C 14:0 2-OH (11.3%), and Summed Features 8 (46.6%). Phylogenetic, genomic and phenotypic analyses suggest that strain sgz301304 T belongs to a novel species within the genus of Sphingobium . Therefore, it is recommended to be named as Sphingobium agrisoil sp. nov. The type strain is sgz301304 T (= MCCC 1K09180 T  = JCM 37704 T ), respectively.