Protocol for the synthesis and characterization of Ru@PCN@PEI nanoparticles with superoxide dismutase catalytic activity

Bimetallic organic frameworks (BMOFs) possess versatile structural and functional features that enable applications in catalysis and biomedicine. Here, we present a protocol to synthesize Ru@PCN@PEI nanoparticles by loading Ru nanoparticles onto PCN-222(Mn) and coating the surface with polyethyleneimine (PEI). We describe steps for precursor preparation, particle size regulation, and surface modification. We detail procedures for morphological characterization by transmission electron microscopy and assessment of catalytic activity using ultraviolet-visible (UV-vis) spectroscopy. This protocol enables the preparation and functionalization of BMOFs for advanced nanobiomaterial applications.