Room-temperature synthesis of fluorine-anion engineered cerium oxyfluoride nanozymes via valence-regulation strategy for prompt uric acid biosensing

The catalytic activity of Ce-based nanozymes is significantly positive-correlated with the Ce 3 + ratio. In this work, Ce 3+ content in cerium oxyfluoride nanozymes was efficiently and directly regulated with fluorine-induced charge compensation between fluorine and oxygen, and stabilized by the electronegativity-driven inductive effect, through the facile and room-temperature synthesis for the enzyme /H 2 O 2 -free and highly sensitive UA detection. Remarkably, cerium oxyfluoride exhibited V max of 196 × 10 −8 M·s −1 and K m of 0.347 mM, indicating the superior oxidase-like activity. Thereby, ascribed to the antioxidant property of uric acid (UA), UA was effectively and promptly detected, i.e., a wide linear range of 0.3–1.2 mM, and ultra-low detection limit of 4.5 μM, as well as the significant anti-interference against the common urinary components. Prospectively, the electron-density reforming strategy through the strong electronegative fluorine introduction, extremely regulated the valence of cerium in cerium oxyfluoride to enhance the catalytic activity, via the synthetic route of mildness and facileness, fulfilling the effective real-time detection of UA.