AIE-active UiO-66@PtNPs nanozyme-amplified fluorescence and colorimetric dual-signal magnetic immunosensing platform for effective detection of aflatoxin B1 in food products

Highly toxic aflatoxin B 1 (AFB 1 ) frequently contaminates food products, posing a serious threat to human health. Developing accurate, efficient and user-friendly detection strategies is essential. This study synthesized a novel H 4 TCPE-UiO-66@PtNPs nanozyme with stable fluorescence and catalytic properties through integrating H 4 TCPE ligands possessing aggregation-induced luminescence (AIE) properties with in-situ grown PtNPs, and further developed a fluorescence-colorimetric dual-signal immunosensing platform for the detection of AFB 1 contamination in food products. Utilizing an immunocompetitive reaction and magnetic separation techniques, the platform enabled quantitative analysis of AFB 1 , achieving favorable linear range of 0.05–100 ng/mL and high sensitivity (fluorescence LOD: 0.022 ng/mL, colorimetric LOD: 0.047 ng/mL). Real samples tests yielded satisfactory recoveries (84.6%–110.1%) and good reproducibility (RSD ≤ 5.3%, n  = 3), demonstrating the platform's potential for specific identification and screening of AFB 1 in complex matrices. The proposed dual-signal detection strategy provides an effective solution for self-calibrated and rapid analysis of foodborne or environmental pollutants.