Promoting effect of peroxidase-like activity covalent organic frameworks on luminol-H2O2 cathodic electroluminescence and its sensing application

Two covalent organic framework (COF) materials—TAPA-TFPB-COF and TAPA-BTT-COF—were synthesized through Schiff-base condensation reactions, and their catalytic performance in the luminol-H 2 O 2 cathodic electrochemiluminescence (ECL) system was systematically investigated. The results demonstrated that TAPA-BTT-COF exhibited superior peroxidase-like activity, which could effectively catalyze the decomposition of H 2 O 2 to generate reactive oxygen species (ROS) and significantly enhance the ECL intensity. Based on this catalytic mechanism, a highly sensitive and selective method for the detection of tyrosine (Tyr) was established, with a linear detection range from 1.0 × 10 − 8 to 1.0 × 10 − 4 mol L − 1 and a detection limit as low as 6.2 × 10 − 10 mol L − 1 . This study innovatively explored and utilized the intrinsic enzyme-like activity of COF materials as a substitute for traditional biological enzymes to construct a stable, cost-effective, and ultrasensitive enzyme-free ECL sensing platform, which might further expand the biosensing application of COF materials.