On-Site Multiplex Detection of Foodborne Bacteria Using a Smartphone-Assisted Magnetic MOF-RPA-CRISPR Platform

The rising global burden of foodborne illnesses necessitates the development of rapid, sensitive, and multiplex detection technologies for on-site screening. This study presents an innovative smartphone-assisted magnetic MOF-RPA-CRISPR platform for the simultaneous detection of four major foodborne pathogens: Salmonella Typhimurium , Staphylococcus aureus , Vibrio parahaemolyticus , and Listeria monocytogenes . The platform’s originality lies in the one-pot integrated workflow of three functional modules: a core-shell structured Fe₃O₄@UiO-66-NH₂ material for efficient bacterial enrichment with over 90% capture efficiency, a quadruplex RPA-CRISPR/Cas12a assay enabling specific amplification and detection with a sensitivity of 10 CFU/mL, and an intelligence visual system that provides quantitative, objective readouts. When validated with spiked food samples including milk, chicken, and shrimp, the entire assay was completed within 60   minutes without specialized equipment, demonstrating high accuracy and robustness against matrix interference. This integrated approach offers a powerful and user-friendly solution for rapid, on-site multiplex pathogen monitoring, significantly advancing food safety surveillance capabilities.