Design of persistent luminescent nanoenzyme patches for dual-mode detection of lung cancer biomarkers aniline and o-toluidine

For the first time, the MnO 2 @MgGa 2 O 4 composite material was made and synthesized. • Using MgGa 2 O 4 phosphorescence and MnO 2 peroxidase activity, aniline and o-toluidine can be detected simultaneously. • On-site aniline and o-toluidine testing with a mobile analysis tool on a smartphone. • The wearable patch sensor makes it easy to detect aniline and o-toluidine. Aniline and o-toluidine are crucial biomarkers for lung cancer, so their detection becomes significant for healthcare. Here we developed a stable, accurate, and sensitive wearable nanozyme patch based on MnO 2 @persistent luminescent nanoparticles (MnO 2 @PLNPs) for the detection of aniline and o-toluidine. MgGa 2 O 4 :0.1%Zn 2+ (MZGO) with the emission at 710 nm were first prepared and then loaded MnO 2 nanozyme as MnO 2 @PLNPs. The composite was embedded in polyvinyl alcohol (PVA) matrix to obtain the wearable nanozyme patch. MnO 2 exhibit broad band absorption in 300–800 nm for quenching the red phosphorescence of PLNPs. Upon the introduction of aniline and o-toluidine, MnO 2 nanozyme reacts with both analytes and degrades as Mn 2+ . Thus, the long-afterglow emission of PLNPs is recovered and this dual change enables detection of aniline and o-toluidine with a detection limit of 0.9 mmol with the phosphorescence at 710 nm and color change. Additionally, analyzing the RGB values of the reaction using a smartphone allows in-site determination of aniline and o-toluidine for early alert of lung cancer. Download: Download high-res image (117KB) Download: Download full-size image