An electromagnetically driven fluorescence sensing platform integrated with a smartphone for simultaneous detection of chloramphenicol and oxytetracycline

Synchronous detection method for chloramphenicol and oxytetracycline. • Integrating electromagnetically driven fluorescence visual sensing platform with a smartphone. • Signal separation of dual-emission AuNCs by different magnetic response of MNPs. In this work, a synchronous detection method for chloramphenicol (CAP) and oxytetracycline (OTC) was developed by integrating electromagnetically driven fluorescence visual sensing platform with a smartphone. Typically, red-AuNCs and green-AuNCs were used as fluorescence signal probes to respectively bind CAP and OTC aptamers, and strong- and weak- magnetic nanoparticles (S-MNPs and W-MNPs) combining with the corresponding complementary chain were functioned as capture probes. Based on the characteristics of dual-emission AuNCs, synchronous and sensitive detection of CAP and OTC can be achieved by fluorescence. Further, due to the different magnetic response of MNPs, red-AuNCs and green-AuNCs were separated in the electromagnetically driven zonal chip platform, fluorescent visual detection of CAP and OTC was achieved by coupling with a smartphone. The fluorescence detection limits of the constructed method for CAP and OTC were 0.008 μmol/L and 0.005 μmol/L respectively, and the fluorescence visualization detection limits were 0.075 μmol/L and 0.037 μmol/L. Download: Download high-res image (192KB) Download: Download full-size image