Dual-site Cu/Zn-MOF nanozyme orchestrates the sequential healing of infected wounds

Cu/Zn-MOF nanozyme exhibits robust triple-enzyme activities. • It promotes healing by combining antibacterial, antioxidant, and pro-proliferative effects. • This work provides a strategy for designing multifunctional nanoenzyme platform for efficient wound therapy. The treatment of infected wounds is severely challenged by bacterial resistance and dysregulated healing processes. Herein, we report a bimetallic Cu/Zn-MOF nanozyme fabricated via a facile self-assembly strategy. The material exhibits robust triple-enzyme mimetic activity (superoxide dismutase (SOD), glutathione peroxidase (GPx), and ascorbate peroxidase (APx)) enabling efficient reactive oxygen species (ROS) scavenging to alleviate oxidative stress and inflammation. In vitro, Cu/Zn-MOF demonstrated potent, broad-spectrum antibacterial activity, primarily associated with disruption of bacterial nucleotide metabolism and energy homeostasis, while also promoting fibroblast proliferation and migration. In an infectious wound model, Cu/Zn-MOF treatment significantly accelerated wound closure, enhanced collagen deposition, and facilitated orderly tissue regeneration, achieving a healing rate of 93.4% by day 12. This work presents a versatile nanozyme platform that intelligently orchestrates the sequential stages of wound healing, offering a promising multi-functional strategy for regenerative medicine.