A novel fluorescent probe for real-time in vivo imaging of H2O2 level in polycystic ovary syndrome

This work presents LH, a hemicyanine-based activatable fluorescent probe for the sensitive detection of hydrogen peroxide (H 2 O 2 ). LH undergoes H 2 O 2 -triggered cleavage of the boronate recognition unit, resulting in a turn-on near-infrared (NIR) fluorescence response. The probe exhibited favorable sensitivity, with a limit of detection (LOD) of 0.04 μM, as well as good selectivity and biocompatibility under the tested conditions. Cellular imaging experiments demonstrated that LH could monitor intracellular H 2 O 2 changes in ovarian-derived cells. Furthermore, LH was applied to PCOS-model mice and serum samples, where enhanced fluorescence signals were observed compared with healthy controls, suggesting increased H 2 O 2 -associated oxidative changes in the PCOS model. These results indicate that LH may serve as a useful molecular imaging tool for studying oxidative stress-related changes in PCOS-model mice.